Preparation of reagents

Copper Sulphate solution specific gravity 1053)

This solutionisused for haemoglobin estimation of blood donors. The methodis based on specific gravity and is reasonably reliable method for determining the haemoglobin of blood donors. It is indirect method of the Hib values.


Preparation of copper sulphate solution:
 CuSO4 solution of sp. Gr. 1.053
Method -1:

 Prepare stock solution of copper sulphate (sp. Gravity 1.100)
► Dissolve 159.63 of pure air dried crystals of copper sulphate (CuSO4.5H2O) in distilled water and make up to exactly 1000 ml at 25°C. The specific gravity of the solution must be 1.100. –
. Preparation of CuSO4Solution of specific gravity 1.052-1.055
Sp Gr
Stock Solution
Distilled Water
Hb Equivalent
1.052
51 ml
100ml
12.0 g
1.053
52ml
100ml
12.5g
1.054
53ml
100ml
13.0g
1.055
54ml
100ml
13.4g

The solution should be stored at room temperature in tightly capped containers to prevent evaporation. - -


Method-2:
► Dissolve 8.33 gm of pure airdried crystals of copper sulphate in 100 ml distilled water. The specific gravity of the solution must be 1.053





Quality control:
1.     Every batch of CuSO4 should be checked with blood samples of known haemoglobin in a range around 12.5g/dl., (eg. 12g/dl;12.5g/dl; 13.0g/dl and 13.5g/dl).
2.    Gently place a drop of each blood sample into CuSO4 solution of sp. gravity 1,053
3.    Drops of all Hb of 12.5 d/l or above will sink and those with Hb level below 12.5 g/dl will float. -


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