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Unexpected antibody test

Saline method of antibodies testing Method: 1.     Put 2 drops oftest serum in a labeled tube 2.     Add 1 drop of 2-4% suspension of appropriate red cells in Saline and mix 3.     Incubate at R.T for 30-45 min. In urgent cases centrifuge the tubes at 1000 rpm for 1 min after 5-10 min. incubation at room temperature (spin method) 4.     Observe the supernatant fluid for the presence of haemolysis 5.     Gently disperse the cell button and check for agglutination against a well lighted background Albumin method of antibodies testing Method: 1.     Add 2 drops oftest serum to a labeled tube 2.     Add one drop of 2-4% suspension of appropriate RBC in Saline 3.     Add two drops of bovine albumin 22% 4.     Mix and incubate at 37°C for 30-60 minutes 5.     Centrifuge at1000 rpm for 1 minutes 6.    ...
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T.T.D TESTING

HIV Elisa test :  Assay procedure:    Bring all the reagents to room temperature   Arrange required no. of wells and proceed   25µl of conjugate -1 to each well (ready to use 75 µl of negative control (R3) in well1A1 75µl of cut off control (R4) in wells B1, C1, at D1   75µl of HIV positive control (R5) in well E1 & F1   75 µl of test sample to other remaining wells Mix well, seal with adhesive film and incubate at 37Cfor 1 hour   Wash 3 times with working wash solution (Concentrate - R2 100 ml+Dist. Water 1000 ml) 100 µl of working conjugate-2 to each well (Working conjugate-2 = Mix conjugate-R7a Feojugate dialent-R7b) Seal and incubate at room temperature for 30 miles   Wash 5 times   80µl fresh working development solution (Chromogen-R91ml+ Substrate buffer Rs.10 ml Incubate for 30 minutes at dark place. 100 µl stopping solution (ready to use) Read at 450 & 620 after 4 minutes ...
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Storage of Blood and Blood Products.

Srl No   Name of the product Temperature Shelf Life 1. Whole Blood ( CPDA ) 2 -6 *c 35 Days 2. (a) Concentrated RBC ( CPDA-1) closed system (b) Concentrated RBC ( CPDA-1) open system (c) Concentrated RBC ( SAGM) 2 -6 *c 2 -6 *c 2 -6 *c 35 Days 24 Hrs 42 Days 3. Fresh Frozen Plasma -30*c 1 year 4. Thawed 2 -6 *c 12 Hrs 5. Platelet concentrate 20 – 24 *c 3- 5 days (Depending on the bag ) Other items to be observed     Pilot tubes of issued blood                            -         ...
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REGISTERS FOR BLOOD BANK

. DONOR MASTER RECORD Srl no./Date of donation/Name ofdonor/Age/Sex/Address/Phone no/ Occupation/weight/Hb/BP/Pulse/Donor no/Tube no./Date of expiry / component prepared(yes or no)/Bagtype/Quantity in ml/Type of donor(Voluntary or Replacement/If replacement patient name/ Time of phlebotomy/Any donor reaction/HIV status/HBV status/HCV status/Syphilis status/Malaria status/Antibody status/if positive titre/identification of antibody/signature of the M.O 2.DONOR REGISTRATION RECORD  Sr. No/Date of donation/Name of the donor/Address/Age/Sex/date of donation/type of donor/ Signature of receptionist/ Signature of donor 3. DONOR DEFERREL REGISTER  Srl. No/Date of donation/Name of donor/address/Age/Sex/Type of donor/Cause for deferral/Permanent or temporary/signature of M.O 4. DONOR REFERRAL REGISTER Srl. No/Date/Date of donation/Name of donor/address/Age/Sex/Type of donor Seroreactive for/referred to/Name of ICTC/signature of M.O 5. PATIENT REGISTRATIO...
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